If you don't remember your password, you can reset it by entering your email address and clicking the Reset Password button. You will then receive an email that contains a secure link for resetting your password
If the address matches a valid account an email will be sent to __email__ with instructions for resetting your password
We followed–up a mild COVID-19 patient for 91 days and serially monitored his serum antibodies to four SARS-CoV-2 related antigens (NP, RBD, S1 and ECD) and neutralization activities. Our data revealed a profile of serial antibody responses during the progress and a quick decline of neutralization activities after discharge.
Since December 2019, an outbreak of 2019 novel coronavirus disease (COVID-19) causing a severe pneumonia has been spreading globally [
]. Though, recent studies demonstrated that transfusion of convalescent plasma containing the neutralizing antibody responses resulted in clinical improvement [
]. The isotype, specificity and duration of protective antibodies in recovered COVID-19 patients were still not well-known. Antiviral treatment, infection control, epidemiological measures, and vaccination against SARS-CoV-2 were in urgent demand of reliable data for profiles of serial serum antibody responses during the progress of COVID-19. Here we retrospective studied a longitudinal profile of anti-SARS-CoV-2 antibody responses in a COVID-19 patient with mild clinical presentation. We serially monitored the IgA, IgM, IgG and IgG isotypes including IgG1 to IgG4 responses specific to four SARS-CoV-2 related antigens, including nucleocapsid protein (NP) and receptor binding domain (RBD), S1 protein, and ectodomain of spike protein (ECD) [
Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study.
], from the 4th day he was symptom onset till the 91st day after symptom onset.
On February 1, 2020, a 27-year-old man sought medical advice for a fever of 38 °C and cough. Upon admission, his chest computed tomography (CT) scans showed focal ground-glass opacities and nasopharyngeal swab test was positive for SARS-CoV-2 by real-time reverse transcription-PCR (RT-PCR). He was diagnosed as mild symptomatic patients and admitted to the hospital. The RT-PCR tests for SARS-CoV-2 were positive for two days and symptoms were resolved except for mild cough in the next following days. For all RT-PCR results were negative since February 4, he was discharged on February 22. And we followed him up till the 91st day after symptom onset. The clinical course was summaried (Fig. 1A).
Fig. 1The clinical course and the SARS-CoV-2 specific antibody responses in a COVID-19 patient with mild presentation. (A) Timeline of clinical symtoms, chest radiography findings and qRT-PCR results for the COVID-19 patient. His-cough was gradually allievated since Febuary 7, and chest radiographic improvement was observed on Febuary 12 and Febuary 16, respectively. (B) Longidudial IgA, IgM, IgG, IgG1 and IgG3 antibody titers in response to SARS-CoV-2 nucleocapsid protein (NP) and various subunits of spike protein including receptor binding domain (RBD), S1, and ectodomain (ECD), respectively. (C) Serial monition of serum antibodies neutralization activities of from January 29 to May 1, 2020.
Using four recombinant SARS-CoV-2 antigens, serial specific IgA, IgM, IgG and IgG isotypes including IgG1 to IgG4 responses were analyzed by an indirect enzyme-linked immunosorbent assay (ELISA) (Fig. 1B). Of note, the level of anti-SARS-CoV-2 IgG2 and IgG4 were almost undetectable. IgG specified to all the four antigens were peaked at 27 days after symptoms onset and decreased gradually until the 91 days after symptoms onset. Correlatively, IgG1 specified to ECD, S1 and RBD were peaked at 7 days and specified to NP was peaked at 14 days after symptoms onset. And IgG1 specified to all the four antigens stayed at relative high level till the 91st day. Though IgG3 responses to ECD, S1 and NP were increased since the 4th day and mildly declined until the 91st day, responses especially to RBD was almost undetectable on the 91st day. IgA reacted to ECD and RBD were increased from the 4th day and stayed sustainably at high level until the 91st day. On the contrary, NP and S1 specified IgA dropped rapidly after the peak point and was undetectable at 91st day after the symptoms onset. In addition, NP reacted IgM sharply decreased from 7th day till 17th day and stayed undetectable.
The neutralization activities were further determined by the pseudovirus microneutralization assay. The activity was rapidly increased from the 4th day to the 20th day after the symptoms onset, peaked with a titer of 2954 (ID50), and decreased obviously then. On the 91th day the titer was 114 (ID50), only 4% of the peak point (Fig. 1C).
Our case highlighted that the SARS-CoV-2 specific humoral immunity is critical during clinical recovery of COVID-19. Of note, antibody specific to RBD which is responsible for binding to angiotensin-converting enzyme 2 (ACE2) was correlated with neutralizing capability [
]. Early presence of anti-RBD antibody might facilitate virus clearance, contributing to a transient positive viral detection. The level of RBD-specific antibody might be associated with the favorable outcome of COVID-19 [
]. Secondly, high magnitude of antibody responses targeting at spike protein RBD region was identified, suggesting that RBD region is highly immunogenic, an ideal antigen candidate for vaccine design. Thirdly, our data suggested a rapidly declined neutralizing activity of COVID-19 clinical recovered patients 69 days after discharge, suggesting the circulating anti-SARS-CoV-2 neutralizing antibodies might have a relative short half-life. Fourth, this case validated the necessities of the combination of SARS-CoV-2 specific antibody responses with viral testing for diagnosis, especially for patients with minimal or mild presentation [
]. Such information is of immediate relevance and would assist clinical diagnosis, prognosis and vaccine design of COVID-19.
Acknowledgment
This study was supported by the Medical Science and technology Development Foundation, Nanjing Department of Health (Grant No. YKK19056), Nanjing Medical Science and Technique Development Foundation (QRX17141), Fundamental Research Funds for the Central Universities (No. 14380459).
References
Zhu N.
Zhang D.
Wang W.
Li X.
Yang B.
Song J.
et al.
A novel Coronavirus from patients with Pneumonia in China, 2019.
Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study.
30457-6&id=gr1.jpg){kind=link}