Highlights
- •Genome sequencing of 43 measles virus isolates from 10 Indian states sampled during 2009–2017.
- •MeV named strains for one D4 and two D8 isolates were identified based on N-450.
- •D4 isolates have non-standard genome length due to indels in M-F intergenic region.
- •Genotype-based temporal clustering devoid of geography-based monophyly was observed.
- •Observed variations may not impact antigenicity and support use of current vaccines.
Summary
Objective
To map genomic diversity of Measles virus (MeV) isolates collected during 2009–2017
from ten states of India.
Methods
Genome sequencing of Indian isolates and comparative genomics with global MeV using
phylogeny, population stratification and selection pressure approaches were performed.
Results
The first report of complete genome sequences of forty-three Indian MeV isolates belonging
to genotypes D4 (eight) and D8 (thirty-five). Three Indian isolates mapped to named
strains D4-Enfield, D8-Villupuram and D8-Victoria. Indian D4 isolates deviate from
standard genome length due to indels in M-F intergenic region. Estimated nucleotide
substitution rates of Indian MeV derived using genome and individual genes are lower
than that of global isolates. Phylogeny revealed genotype-based temporal clustering,
suggesting existence of two lineages of D4 and three lineages of D8 in India. Absence
of spatial clustering suggests role of cross-border travel in MeV transmission.
Conclusions
Evolutionary analyses suggest the need for surveillance of MeV in India, particularly
in view of diversified trajectories of D4 and D8 isolates. This study contributes
to global measles epidemiology and indicates no major impact on antigenicity in Indian
isolates, thereby substantiating the use of current vaccines to meet measles elimination
target of 2023 set by World Health Organization for South-East Asia Region.
Keywords
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Article info
Publication history
Published online: January 17, 2020
Accepted:
November 30,
2019
Identification
Copyright
© 2020 The British Infection Association. Published by Elsevier Ltd. All rights reserved.
