Conventional methods for diagnosing CRBSI such as quantitative or semiquantitative catheter-tip cultures require removal of the CVC. However, CVCs removed on suspicion of CRBSI prove to be the source of blood stream infection in only 15% of cases.
1It has been shown that clinically suspected CRBSI can be detected by the differential time to positivity (DTP) method or Gram/acridine-orange leukocyte cytospin (AOLC) test without catheter removal.
- Bong J.J.
- Kite P.
- Ammori B.J.
- Wilcox M.H.
- McMahon M.J.
The use of a rapid in situ test in the detection of central venous catheter-related bloodstream infection: a prospective study.
J Parenter Enteral Nutr. 2003; 27: 146-150
- Chen W.T.
- Liu T.M.
- Wu S.H.
- Tan T.D.
- Tseng H.C.
- Shih C.C.
Improving diagnosis of central venous catheter-related bloodstream infection by using differential time to positivity as a hospital-wide approach at a cancer hospital.
J Infect. 2009; 59: 317-323
3Whether microorganisms can be detected in blood drawn from CVCs before symptoms of CRBSI become manifest using the Gram/AOLC method has not been investigated. The aim of this prospective pilot study was to investigate whether the Gram stain/AOLC method might be a potentially useful way to anticipate the diagnosis of CRBSI.
- Kite P.
- Dobbins B.M.
- Wilcox M.H.
- McMahon M.J.
Rapid diagnosis of central-venous-catheter-related bloodstream infection without catheter removal.
Lancet. 1999; 354: 1504-1507
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- The use of a rapid in situ test in the detection of central venous catheter-related bloodstream infection: a prospective study.J Parenter Enteral Nutr. 2003; 27: 146-150
- Improving diagnosis of central venous catheter-related bloodstream infection by using differential time to positivity as a hospital-wide approach at a cancer hospital.J Infect. 2009; 59: 317-323
- Rapid diagnosis of central-venous-catheter-related bloodstream infection without catheter removal.Lancet. 1999; 354: 1504-1507
- Detection of catheter-related bloodstream infections by the differential-time-to-positivity method and gram stain-acridine orange leukocyte cytospin test in neutropenic patients after hematopoietic stem cell transplantation.J Clin Microbiol. 2004; 42: 4835-4837
- Delayed processing of blood samples influences time to positivity of blood cultures and results of Gram stain-acridine orange leukocyte Cytospin test.J Clin Microbiol. 2007; 45: 2691-2694
- Diagnosis of central venous catheter-related sepsis. Critical level of quantitative tip cultures.Arch Intern Med. 1987; 147: 873-877
- Intravascular catheter-related infections: advances in diagnosis, prevention, and management.Lancet Infect Dis. 2007; 7: 645-657
- Increased resource use associated with catheter-related bloodstream infection in the surgical intensive care unit.Arch Surg. 2001; 136: 229-234
- Evaluation of outcome of intravenous catheter-related infections in critically ill patients.Am J Respir Crit Care Med. 2000; 162: 1027-1030
Published online: January 25, 2010
Accepted: January 4, 2010
© 2010 The British Infection Society. Published by Elsevier Inc. All rights reserved.