Conventional methods for diagnosing CRBSI such as quantitative or semiquantitative
catheter-tip cultures require removal of the CVC. However, CVCs removed on suspicion
of CRBSI prove to be the source of blood stream infection in only 15% of cases.
1
It has been shown that clinically suspected CRBSI can be detected by the differential
time to positivity (DTP) method or Gram/acridine-orange leukocyte cytospin (AOLC)
test without catheter removal.
2
,
3
Whether microorganisms can be detected in blood drawn from CVCs before symptoms of
CRBSI become manifest using the Gram/AOLC method has not been investigated. The aim
of this prospective pilot study was to investigate whether the Gram stain/AOLC method
might be a potentially useful way to anticipate the diagnosis of CRBSI.To read this article in full you will need to make a payment
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References
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- Delayed processing of blood samples influences time to positivity of blood cultures and results of Gram stain-acridine orange leukocyte Cytospin test.J Clin Microbiol. 2007; 45: 2691-2694
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- Intravascular catheter-related infections: advances in diagnosis, prevention, and management.Lancet Infect Dis. 2007; 7: 645-657
- Increased resource use associated with catheter-related bloodstream infection in the surgical intensive care unit.Arch Surg. 2001; 136: 229-234
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Article info
Publication history
Published online: January 25, 2010
Accepted:
January 4,
2010
Identification
Copyright
© 2010 The British Infection Society. Published by Elsevier Inc. All rights reserved.
