Summary
Objective
Multi-drug resistance and latent infection are two major issues in current tuberculosis
(TB) control and management. Capreomycin is an important drug used for TB with multi-drug
resistance. A recent study also indicates that this drug possesses unique bactericidal
activity against non-replicating TB bacilli among known anti-TB drugs. Thus, there
is an urgent need for investigating the full-spectrum action of capreomycin.
Methods
Here we conduct the first microarray-based study on capreomycin using the high-resolution Affymetrix
oligonucleotide GeneChip system.
Results
The results indicate that capreomycin primarily acts on the information pathways but
it also significantly affects cell wall, cell processes, intermediate metabolism and
respiration in Mycobacterium tuberculosis.
Conclusions
This study not only transcriptionally validates the specific molecular target, 16S
rRNA, but also discovers potential new targets of capreomycin, including genes operating
at the DNA level, such as Rv0054 (ssb) and Rv3715c (recR), as well as genes involved in cell division like Rv3260c (whiB2). In addition, the nuo gene cluster and the ATP synthase gene cluster are repressed.
Keywords
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Article info
Publication history
Accepted:
May 24,
2006
Identification
Copyright
© 2006 The British Infection Society. Published by Elsevier Inc. All rights reserved.
