Abstract
This study investigates the action of Canova medication (CM) on experimental infection
by Leishmania (Leishmania) amazonensis, utilizing in vitro and in vivo assays. For the in vitro tests, Balb/c mouse peritoneal
macrophages (5×105 cells in 500 μl of culture medium, supplemented with 10% fetal calf serum, penicillin (100 U/ml) and streptomycin (0.1 mg/ml) (were distributed in 24-well plates and CM was added at concentrations of 20
or 40%. Twenty-four hours later, the macrophages were infected with Leishmania amastigotes in culture medium. The effect of CM on macrophages leishmanicidal activity
in 24 and 48 h cultures was evaluated by determining infection index and measuring nitric oxide
(NO) production. The in vivo tests were performed in mice infected with 107 L. (L.) amazonensis promastigotes injected in to the right hind footpad (25 μl in phosphate buffered saline). The progression of the lesions was examined over
a 9-week period by measuring footpad swelling, and the parasite load in regional lymph
nodes and spleen. The in vitro results showed that at 40% CM reduced the infection
index, and induced NO production in the elicited macrophages, which suggests that
the inhibitory effect on infection index may be mediated by NO. In the in vivo infection,
when administered, orally or subcutaneously in mice, CM reduced infection by L. (L.) amazonensis in the paws, resulting in smaller lesions. CM treatment also decreased parasite load
in the regional popliteal lymph nodes and in the spleen. These results suggest that
CM modulates experimental infection by L. (L.) amazonensis, controlling infection progression and limiting dissemination.
Keywords
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Article info
Publication history
Accepted:
September 12,
2004
Identification
Copyright
© 2004 The British Infection Society. Published by Elsevier Inc. All rights reserved.
