Abstract
Objectives. To study the action of factors produced by living Campylobacter jejuni (C. jejuni) against those present within sonicated and filtrated bacteria on induction of potential
cytokines by the human intestinal cell line INT407.
Methods. We used immunohistochemical technique modified to detect intracellular production
of cytokines protein and RT-PCR to read RNA messages for evaluation of de novo cytokine
synthesis.
Results. The data herein display dissociation of cytokine profiles induced on by living C. jejuni. Exposure of INT407 cells to 106 live bacteria showed the highest numbers of cytokine producing cells of all examined
cytokines. IFN-γ was the highest induced cytokine followed by IL-10, TNF-α and lastly
IL-4. Also, abrogation of induction of the proinflammatory cytokines IFN-γ and TNF-α
but not the antiinflammatory cytokines IL-4 and IL-10 by sonicated and filtrated bacteria
was depicted. At the mRNA level, TNF-α signals were noted in accordance with its protein
levels since increased TNF-α mRNA signals were registered only after stimulation with
living bacteria. Very low or no induction of TNF-α was registered with non-stimulated
cells.
Conclusions. These results illustrate for the first time a role for factors from living bacteria
in directing the immune response towards Th1 type. Characterization of such factors
may be essential for future immunotherapeutic interventions during severe bacterial
infections.
Keywords
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Article info
Publication history
Accepted:
May 11,
2003
Identification
Copyright
© 2003 The British Infection Society. Published by Elsevier Inc. All rights reserved.
