Abstract
Objectives. NS1 expression of human parvovirus B19 is a critical event in B19 pathogenesis.
However, the mechanisms of NS1-induced cytotoxicity in B19 infection have yet to be
clarified mainly because of the absence of multifunctional monoclonal antibodies (Mab)
against NS1. The purpose of this study was to establish such Mab, which function in
immunoprecipitation assays, immunoblotting, indirect immunofluorescence, and immunohistochemical
staining.
Methods. Balb/c mice were immunized with the recombinant NS1 protein and hybridoma cell lines
producing Mab against the NS1 protein were screened by ELISA, immunoprecipitation,
immunoblotting, and indirect immunofluorescence staining. The Mab were used for immunohistochemical
staining of formalin-fixed tissues from an intrauterine B19 infected fetus.
Results. ParC-NS1, the monoclonal antibody against the NS1 protein, worked in all tested
screening methods. ParC-NS1 could also detect NS1 protein expressed in clinical tissue
specimens.
Conclusions. The ParC-Ns1 monoclonal antibody was specific against the NS1 protein. In addition,
NS1 protein expression was successfully identified in human tissues. These data indicated
that this monoclonal antibody should be a useful tool to study the kinetics and sites
of NS1 expression and NS1-induced cytotoxicity in B19 infection.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of InfectionAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Parvovirus-like particles in human sera.Lancet. 1975; I: 72-73
- Human parvovirus, the cause of erythema infectionsum (fifth disease)?.Lancet. 1983; i: 1378
- Parvovirus B19 in human disease.Annu Rev Med. 1997; 48: 59-67
- Erythrocyte P antigen; cellular receptor for B19 parvovirus.Science. 1993; 262: 114-117
- Resistance to parvovirus B19 infection due to lack of virus receptor (erythrocyte P antigen).N Engl Med. 1994; 330: 1192-1196
- Intrauteine parvovirus infection associated with hydrops fetalis.Lancet. 1984; ii: 1033-1034
- Parvovirus B19 infection induces apoptosis of erythroid cells in vitro and vivo.J Infect. 1999; 39: 68-76
- Hematologic and hematopoietic consequences of B19 parvovirus infection.Semin Hamatol. 1988; 25: 159-192
- Propagation of human parvovirus B19 in primary culture of erythroid lineage cell derived from fetal liver.J Virol. 1989; 63: 2422-2426
- The incidence of and factors leading to parvovirus B19-related hydrops fetalis following maternal infection, report of 10 cases and meta-analysis.J Infect. 1998; 37: 28-35
- Immediate and long term outcome of human parvovirus B19 infection in pregnancy.Br J Obstet and Gynaecol. 1998; 105: 174-178
- Novel transcription map for the B19 (human) pathogenic parvovirus.J Virol. 1987; 61: 2395-2406
- Replication of the B19 parvovirus in human bone marrow cell cultures.Science. 1986; 233: 883-886
- Nonstructural proteins of parvovirus B19 and minute virus of mice control transcription.J Virol. 1990; 64: 387-396
- A cytotoxic nonstructural protein, NS1, of human parvovirus B19 induces activation of interleukin-6 gene expression.J Virol. 1996; 70: 8485-8491
- Transactivation of the long-terminal repeat of human immunodeficiency virus type 1 by the parvovirus B19 NS1 gene product.J Gen Virol. 1993; 74: 2011-2014
- The gene encoding the nonstructural protein of B19 (human) parvovirus may be lethal in transfected cells.J Virol. 1988; 62: 2884-2889
- A putative nucleoside triphosphate-binding domain in the nonstructural protein of B19 parvovirus is required for cytotoxicity.J Virol. 1994; 68: 8443-8446
- Human parvovirus B19 nonstructural (NS1) protein induces apoptosis in erythroid lineage cells.J Virol. 1998; 74: 3018-3028
- Possible interactions between the NS-1 protein and tumor necrosis factor alpha pathways in erythroid cell apoptosis induced by human parvovirus B19.J Virol. 1999; 73: 8762-8770
- A transgenic model for non-immune hydrops fetalis induced by the NS1 gene parvovirus B19.J Gen Virol. 2002; 83: 273-281
- Antibodies to the nonstructural protein of parvovirus B19 in persistency infected patients: implications for pathogenesis.J Infect Dis. 1995; 172: 1356-1359
- Antibodies to parvovirus B19 NS-1 protein in infected individuals.J Gen Virol. 1995; 76: 519-527
- Identification of the major structural and non-structural proteins of human parvovirus B19 and mapping of their genes by prokaryotic expressions of isolated genomic fragments.J Virol. 1986; 60: 548-557
- Characterization of capsid and noncapsid proteins of B19 parvovirus propagated in human erythroid bone marrow cell cultures.J Virol. 1987; 61: 2627-2630
- Human parvovirus B19 induces cell cycle arrest at G2 phase with accumulation of mitotic cyclins.J Virol. 2001; 75: 7555-7563
- Characterization of monoclonal antibodies against human parvovirus B19.Microbiol Immunol. 1989; 33: 561-567
- Cell surface phenotypes and expression of viral antigens of various human cell lines carrying human T-cell leukemia virus.Int J Cancer. 1984; 34: 221-228
- Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis.J Virol. 1986; 58: 921-936
Article info
Publication history
Accepted:
May 9,
2003
Identification
Copyright
© 2003 The British Infection Society. Published by Elsevier Inc. All rights reserved.
