Objectives. To develop a sensitive multiplex PCR to detect HCMV, HHV6 and HHV7, to test this PCR on urine specimens sent to the virus diagnostic laboratory and on stored urine samples from HIV-positive patients and their HIV-negative partners and to compare the sensitivity of the multiplex PCR with the diagnostic laboratory's routine service for the detection of HCMV.
Study design. Primers specific for each of the three viruses were combined in a multiplex PCR that was then optimised for sensitivity. This PCR was applied prospectively to 413 unselected routine urine specimens over a 1 year period and retrospectively to 258 urine specimens from 63 HIV-positive patients and 10 HIV-negative partners.
Methods. In the prospective study, the multiplex PCR detected 40 specimens positive for HCMV alone, 10 for HHV6, 3 for HHV7 and 3 with a dual infection of HCMV and HHV6. The sensitivity for HCMV was 93.5% by multiplex PCR compared to 28.3% by culture. HHV6 DNA was detected in 6 neonates (2–21 days) and HHV7 DNA in 2 neonates (4 and 20 days). In the retrospective study of HIV patients, HCMV was the most commonly detected virus (55.6%) compared to HHV6 (7.9%) and HHV7 (4.8%).
Conclusions. The multiplex PCR was significantly more sensitive than non-DNA based procedures for the detection of HCMV. Urine may be a useful non-invasive specimen for the detection of HHV6 and HHV7 and their presence in neonates suggest perinatal transmission or the possibility of in utero infection.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Journal of Infection
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Human herpesvirus 7 is a T-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus.Proc Natl Acad Sci USA. 1992; 89: 10552-10556
- Human herpesvirus 6.Antiviral Res. 1996; 31: 1-21
- Identification of human herpesvirus 6 as a causal agent for exanthem subitum.Lancet. 1998; 1: 1065-1067
- Human herpesvirus-6: neurologic implications of a newly described viral pathogen.J Neurovirol. 1998; 4: 474-485
- Central nervous system complications in human herpesvirus-6 infection.Brain Develop. 2000; 22: 307-314
- Seroepidemiology study of human herpesvirus-6 and 7 in children of different ages and detection of these two viruses in throat swabs by polymerase chain reaction.J Med Virol. 1996; 48: 88-94
- Human herpesvirus-7: another causal agent for roseola (exanthem subitum).J Pediatr. 1994; 125: 1-5
- Human herpesvirus 6.Rev Med Virol. 2000; 10: 155-173
- Multiplex polymerase chain reaction for human herpesvirus 6, human cytomegalovirus and human β-globin DNA.J Virol Meth. 1995; 53: 223-233
- Detection by PCR of HHV-6 and EBV DNA in blood and oropharynx of healthy adults and HIV-seropositives.Lancet. 1990; 335: 1598-1599
- Polymerase chain reaction for rapid detection of ocular adenovirus infection.J Med Virol. 1995; 46: 126-132
- The polymerase chain reaction for detecting adenovirus DNA in formalin-fixed, paraffin-embedded tissue obtained post mortem.J Inf. 1993; 27: 43-46
- Use of recombinant plasmids to investigate the structure of the human cytomegalovirus genome.J Gen Virol. 1982; 59: 111-129
- The genome of human herpesvirus 6: maps of unit-length and concatemeric genomes for nine restriction endonucleases.J Gen Virol. 1991; 72: 157-168
- Detection of human herpesvirus 6 DNAs in samples from several body sites of patients with exanthem subitum and their mothers by polymerase chain reaction assay.J Med Virol. 1995; 46: 52-55
- Clinical features and viral excretion in infant with primary human herpesvirus 7 infection.Pediatrics. 1995; 95: 187-190
- Detection and typing of lymphotropic herpesviruses by multiplex polymerase chain reaction.J Virol Meth. 1999; 79: 9-19
- Prospective study of persistence and excretion of human herpesvirus-6 in patients with exanthem subitum and their parents.Pediatrics. 1998; 102: 900-904
- Herpesvirus type 6 in patients undergoing bone marrow transplantation: serologic features and detection by polymerase chain reaction.Blood. 1994; 83: 3052-3058
- Cytomegalovirus and human herpesvirus 6 both cause viral disease after renal transplantation.Transplantation. 1998; 66: 877-882
- Detection of human betaherpesvirinae in saliva and urine from immunocompromised and immunocompetent subjects.J Clin Microbiol. 1997; 35: 1600-1603
- Congenital infections with human herpesvirus 6.J Inf Dis. 1998; 178: 544-546
- HHV-6 infection during pregnancy and spontaneous abortion.Lancet. 1992; 340: 1289
- Detection of human herpes virus 6 in fetal hydrops.Lancet. 2000; 355: 1519-1520
© 2003 The British Infection Society. Published by Elsevier Inc. All rights reserved.