Abstract
Objectives. To employ a combination of phenotypic and genotypic subspecies typing methods to
aid in an epidemiological investigation of an outbreak of Salmonella bredeney involving ten persons.
Methods. Isolates were characterised by employing antibiogram typing, in addition to two
genotyping techniques, including pulsed field gel electrophoresis (PFGE), random amplification
of polymorphic DNA (RAPD) with two oligonucleotide primers.
Results. An outbreak of gastroenteritis associated with S. bredeney (serovar O:4 H:Lv 1,7) occurred in Belfast, Northern Ireland in November 1997. In
total, ten cases were confirmed, of which eight had consumed chicken cooked at local
butchers and retailed through one of two local bakeries. One of the remaining cases
was secondarily infected within her home and the final case had eaten a product other
than cooked chicken from one of the bakeries. Food preparation practices were inadequate
in one of the bakeries in question and record keeping and possibly cooking procedures
were inadequate in the butchers. S. bredeney was isolated from an uncooked chicken supplied to the butchers confirming that improperly
cooked chicken was most likely the source of the outbreak. All outbreak clinical isolates
were indistinguishable from each other and were similar to the isolate obtained from
the uncooked poultry demonstrating that these DNA-based methods were valuable in the
molecular characterization of S. bredeney.
Conclusions. This report emphasises the importance and maintenance of an effective hazard analysis
critical control point (HACCP) approach to the processing and retailing of foodstuffs
containing chicken in order to help eliminate hazards to public health.
Keywords
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© 2003 The British Infection Society. Published by Elsevier Inc. All rights reserved.
