Abstract
Objectives. Chlamydia has to replicate in cytoplasmic vacuoles of eukaryotic cells. To understand
how chlamydia interacts with host cells during cell division, we examined the effect
of chlamydial infection on host cell cycle.
Methods. An immunofluorescence microscopic approach was used.
Results. Although the number of cells was significantly reduced in chlamydia-infected cultures,
multiple nuclei were detected in the infected cells, indicating that chlamydia inhibited
host cell growth by selectively blocking cytokinesis while allowing mitosis to proceed.
The chlamydial inhibition of host cell cytokinesis was confirmed with various strains
of Chlamydia trachomatis and in several types of host cells. Furthermore, the chlamydial inhibitory effect
was found to depend on chlamydial protein synthesis.
Discussions. These observations suggest that chlamydia may have evolved specific mechanisms for
actively blocking host cell cytokinesis.
Keywords
Abbreviations:
CPAF, chlamydial proteasome-like activity factor (), MOI, multiplicity of infection ()To read this article in full you will need to make a payment
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© 2003 The British Infection Society. Published by Elsevier Inc. All rights reserved.
