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Anti-Septicaemic Effect of Polysaccharide from Panax ginseng by Macrophage Activation

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      Abstract

      The aim of the present research was conducted to elucidate anti-septicaemic effect of a polysaccharide (PS) isolated from Panax ginseng C.A. Meyer (Araliaceae) by nitric oxide production from stimulated macrophage. In vitro assays for the activity measurement of PS, NO production test with Greiss reagent, phagocytic activity test using zymosan and cytokines production test using ELISA kit were also conducted. In vivo anti-septicaemic activity was assessed by using C57BL/6J mice. This was done with Staphylococcus aureus infection test. PS used at 0.025 mg/kg concentration showed a potent anti-septicaemic activity (80%, survival). However, it did not directly inhibit S. aureus in a minimum inhibitory concentration (MIC) test, conducted in vitro (data not shown). Nitric oxide production via macrophage activation showed the highest value of 5.5 nmol/ml at 1 μg/ml PS. In in vitro phagocytic activity test, PS at 10 μg/ml concentration showed a potent phagocytic activity for zymosan with 167% of the control. Production of TNF-α by macrophage activation at 10 μg/ml of PS was 96% lysis of L929. Also production of IL-1 and IL-6 by stimulation of macrophage with 100 μg/ml PS dose increased to 235 pg/ml and 0.47 ng/ml, respectively. The low mortality of PS treated (0.025 mg/kg) infected mice was concurrent with decreased bacterial content in the blood. Nitric oxide production in S. aureus infected mice whose macrophage was stimulated by PS (0.025 mg/kg) increased approximately 4 times than the untreated S. aureus infected group at 24 and 48 h incubation. In the PS treated (0.025 mg/kg) group, the intracellular concentration of S. aureus in macrophages decreased approximately by 50%, compared with the untreated group. Combine treatment with PS (0.025 mg/kg body weight) and vancomycin (10 mg/kg B.W.) resulted in 100% survival of the animals, whereas only 67% or 50% of the animals survived, respectively, when treated with PS or vancomycin alone. These results suggest that PS from Panax ginseng possess a potent anti-septicaemic activity by stimulating macrophage and a potentiality as an immunomodulator against sepsis occurred byStaphylococcus aureus .
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      References

        • Murphy K
        • Haudek SB
        • Thompson M
        Molecular biology of septic shock.
        New Horiz. 1998; 6: 181-193
        • Rangel-Frausto MS
        The epidemiology of bacterial sepsis.
        Infect Dis Clin North Am. 1999; 13: 299-312
        • Limjoco CM
        • Youmans K
        Reviewing the details of coding septicemia.
        J AHIMA. 2000; 71: 79-80
        • Van der Poll T
        • van Deventer JH
        Cytokines and anticytokines in the pathogenesis of sepsis.
        Infect Dis Clin North Am. 1999; 13: 413-426
        • Michel P
        • Glauser MD
        Pathophysiologic basis of sepsis: Considerations for future strategies of intervention.
        Crit Care Med. 2000; 28: S4-S8
        • Nilsson IM
        • Lee JC
        • Bremell T
        • Ryden C
        • Tarkowski A
        The role of Staphylococcal polysaccharide microcapsule expression in septicemia and septic arthritis.
        Infect Immun. 1997; 65: 4216-4221
        • Hiramatsu K
        • Hanaki H
        • Ino T
        • Yabuta K
        • Oguri T
        • Tenover FC
        Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility.
        J Antimicrob Chemother. 1997; 40: 135-136
        • Hanaki H
        • Kuwahara-Arai K
        • Boyle-Vavra S
        • Daum RS
        • Labischinski H
        • Hiramatsu K
        Activated cell-wall synthesis is associated with vancomycin resistance in methicillin-resistant Staphylococcus aureus clinical strains Mu3 and Mu50.
        J Antimicrob Chemother. 1998; 42: 199-200
        • Klimetzek V
        • Remold HG
        The murine bone marrow macrophage, a sensitive indicator cell for murine migration inhibitory factor and a new method for their harvest.
        Cell Immunol. 1980; 53: 257
        • Flick DA
        • Gifford GE
        Comparison of in vitro cell cytotoxicity assays for tumor necrosis factor.
        J Immunol. 1984; 68: 167-175
        • Nordan PR
        Measurement of human and murine IL-6.
        in: Coligan JE Current Protocols in Immunology, 2nd edn. Greene publishing and Wiley-Interscience, NY1994: 6.6.1-6.6.5
        • Yun YS
        • Lee YS
        • Jo SK
        Inhibition of autochthonous tumor by ethanol insoluble fraction from Panax ginseng as an Immunomodulator.
        Planta Med. 1993; 59: 521-524
        • Lee YS
        • Chung IS
        • Lee IR
        • Kim KH
        • Hong YS
        • Yun YS
        Activation of multiple effector pathways of immune system by the antineoplastic immunostimulator acidic polysaccharide Ginsan isolated from.
        Panax ginseng. Anticancer Res. 1997; 17: 323-332
        • Kim KH
        • Lee YS
        • Jung IS
        • Park SY
        • Chung HY
        • Lee IR
        • Yun YS
        Acidic polysaccharide from Panax ginseng, Ginsan, induces Th1 cell and macrophage cytokines and generates LAK cells in synergy with rIL-2.
        Planta Med. 1998; 64: 110-115
        • Matsuda H
        • Kubo M
        • Tani T
        • Kitagawa I
        • Mizuno M
        Pharmacological study on Panax ginseng C.A. Meyer (XI) Protective effect of red ginseng on infection (2) On phagocytic activity of mouse reticuloendothelial system.
        Shoyakugaku Zasshi. 1987; 41: 135-141
        • Matsuda H
        • Hasegawa T
        • Kubo M
        Pharmacological study on Panax ginseng C.A. Meyer. VII. Protective effect of red ginseng on infection (1) On phagocytic activity of mouse reticuloendotherial system.
        Yakugaku Zasshi. 1985; 105: 948-954
        • Park SH
        • Jo JS
        The effects of Korean ginseng (Panax ginseng C.A. Meyer) extracts and their fractions on the growth of.
        Escherichia coli. Korean J Ginseng Sci. 1993; 17: 203-209
        • Walley KR
        • Lukacs NW
        • Standiford TJ
        Balance of inflammatory cytokines related to severity and mortality of murine sepsis.
        Infect Immun. 1996; 64: 4733-4738
        • Zhao YX
        • Abdelnour A
        • Kalland T
        • Tarkowski A
        Overexpression of the T-cell receptor Vβ3 in transgenic mice increase mortality during infection by enterotoxin A-producing.
        Staphylococcus aureus. Infect Immun. 1995; 63: 4463-4469
        • Cohen J
        Meningococcal disease as a model to evaluate novel anti-sepsis strategies.
        Crit Care Med. 2000; 28: S64-S67
        • Muller Kobold AC
        • Tulleken JE
        • Zijlstra JG
        • Sluiter W
        • Hermans J
        • Kallenberg CGM
        • Cohen Tervaert JW
        Leukocyte activation in sepsis; correlations with disease state and mortality.
        Intensive Care Med. 2000; 26: 883-892
        • Corradin SB
        • Buchmuller-Rouiller YB
        • Mauel J
        Phagocytosis enhances murine macrophages activation by interferon-γ and tumor necrosis factor-α.
        Eur J Immunol. 1991; 21: 2553-2558
        • Fortier AH
        • Polsinelli T
        • Green SJ
        • Nacy CA
        Activation of macrophages for destruction of Francisella tularensis: identification of cytokines, effector cells and effector molecules.
        Infect Immun. 1992; 60: 817-825
        • Malawista SE
        • Montgomery RR
        • van Blaricom G
        Evidence of reactive nitrogen intermediates in killing of staphylococci by human neutrophil cytoplasts.
        J Clin Invest. 1992; 90: 631-636
        • Denis M
        Tumor necrosis factor and granulocyte-macrophage colony-stimulating factor stimulate human macrophages to restrict growth of virulent Mycobacterium avium and to kill avirulent M. avium: Killing effector mechanism depends on the generation of reactive nitrogen intermediates.
        J Leuko Biol. 1991; 49: 380-387
        • Beckerman KP
        • Rogers HW
        • Corbett JA
        • Schreiber RD
        • Mcdaniel ML
        • Unanue ER
        Release of nitric oxide during the T-cell-independent pathway of macrophage function: its role in resistance toListeria monocytogenes.
        J Immunol. 1993; 150: 888-895
        • Green SJ
        • Nacy CA
        Antimicrobial and immunopathologic effects of cytokine-induced nitric oxide synthesis.
        Cur Opin Infect Dis. 1993; 6: 384-396
        • Eizirik E
        Nitric oxide synthase and antimicrobial armature of human macrophages.
        J Infect Dis. 1994; 170: 744-745