Induction of balance and breadth in the immune response is beneficial for the control of SIVmac239 replication in rhesus monkeys

Sun, Caijun; Zhang, Lei; Zhang, Maochao; Liu, Yichu; Zhong, Miao; Ma, Xin; Chen, Ling

doi:10.1016/j.jinf.2010.03.005

« Previous Next »Journal of Infection
Volume 60, Issue 5 , Pages 371-381, May 2010

Induction of balance and breadth in the immune response is beneficial for the control of SIVmac239 replication in rhesus monkeys

Caijun Sun
- Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China
- Graduate School of Chinese Academy of Sciences, Beijing 100049, China
Lei Zhang
- Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China
Maochao Zhang
- Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China
Yichu Liu
- Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China
Miao Zhong
- Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China
- Graduate School of Chinese Academy of Sciences, Beijing 100049, China
Xin Ma
- Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China
Ling Chen
- Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China
- College of life science, University of science and technology of china (USTC), Hefei 230027, China
- Corresponding author. Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, 190 Kai Yuan Avenue, Science Park, Guangzhou 510530, China. Tel.: +86 20 32015295; fax: +86 20 32015299.

Accepted 6 March 2010. published online 29 March 2010.

Summary

Objectives

The aim of this study was to induce cellular and humoral responses with enhanced breadth and more balanced magnitude as a possible approach for an effective HIV vaccine.

Methods

All nine of the SIVmac239 genes (gag, pol, env, nef, vif, vpx, vpr, rev and tat) were optimized for mammalian expression, synthesized and cloned into recombinant adenovirus type 5 (Ad5). These vectors were used as a vaccine regimen, and the immunogenicity and immune protection of this regimen was assessed in murine and macaques.

Results

A vaccine regimen including all nine genes of the SIVmac239 virus was developed, and it was demonstrated that in contrast to single antigen vaccination, the total SIV antigen regimen more effectively elicited the balanced and broad immune responses in murine and macaques. Moreover, the responses afforded effective immune control against infection and replication of the highly pathogenic SIVmac239.

Conclusions

Induction of balance and breadth in the immune response is beneficial in controlling SIVmac239 replication in rhesus monkeys. This study provides insight for the future development of an effective HIV vaccine.

Keywords: HIV vaccine, Immune balance, Immune breadth, Protection, SIVmac239