Journal of Infection
Volume 60, Issue 5 , Pages 344-350 , May 2010

Comparison of same day versus delayed enumeration of TB-specific T cell responses

  • L.M. Lenders

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • R. Meldau

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • R.N. van Zyl-Smit

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • V. Woodburne

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • A. Maredza

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • T.J. Cashmore

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • P.L. Semple

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • M. Badri

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • ,
  • A. Zumla

      Affiliations

    • Centre for Infectious Diseases and International Health, Department of Infection, University College London Medical School, London, UK
    • ,
  • K. Dheda

      Affiliations

    • Lung Infection and Immunity Unit, Division of Pulmonology & UCT Lung Institute, University of Cape Town, Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa
    • Centre for Infectious Diseases and International Health, Department of Infection, University College London Medical School, London, UK
    • Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Department of Medicine, Observatory, Cape Town 7925, South Africa
    • Corresponding Author InformationCorresponding author. Department of Medicine, H floor, Old Main Building, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa. Tel.: +27 21 4066509, (0)845577754 (mobile); fax: +27 21 4486815.

,Accepted 31 January 2010.

References 

  1. Diel R, Loddenkemper R, Meywald-Walter K, Gottschalk R, Nienhaus A. Comparative performance of tuberculin skin test, QuantiFERON-TB-Gold In Tube assay, and T-Spot.TB test in contact investigations for tuberculosis. Chest. 2009 Apr;135(4):1010–1018
  2. Pai M, Zwerling A, Menzies D. Systematic review: T-cell-based assays for the diagnosis of latent tuberculosis infection: an update. Ann Intern Med. 2008 Aug 5;149(3):177–184
  3. Meier T, Eulenbruch HP, Wrighton-Smith P, Enders G, Regnath T. Sensitivity of a new commercial enzyme-linked immunospot assay (T SPOT-TB) for diagnosis of tuberculosis in clinical practice. Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):529–536
  4. T-cell Xtend™ [package insert]. Oxford, England: Oxford Immunotec Ltd.; 2008;
  5. T-SPOT®.TB [package insert]. Oxford, England: Oxford Immunotec Ltd.; 2008;
  6. van Zyl-Smit RN, PM , Peprah K, Meldau R, Kieck J, Juritz J, et al. Within-subject variability and boosting of T cell IFN-γ responses following tuberculin skin testing. Am J Respir Crit Care Med. 2009;
  7. Pai M, O'Brien R. Serial testing for tuberculosis: can we make sense of t cell assay conversions and reversions?. PLoS Med. 2007 Jun 12;4(6):e208
  8. Higgins J, Metcalf JA, Stevens RA, Baseler M, Nason MC, Lane HC, et al. Effects of lymphocyte isolation and timing of processing on detection of CD127 expression on T cells in human immunodeficiency virus-infected patients. Clin Diagn Lab Immunol. 2005 Jan;12(1):228–230
  9. Read SW, Rupert A, Stevens R, O'Shea A, Sereti I. Delayed sample processing leads to marked decreases in measured plasma IL-7 levels. J Acquir Immune Defic Syndr. 2006 Aug 1;42(4):511–512
  10. McKenna KC, Beatty KM, Vicetti Miguel R, Bilonick RA. Delayed processing of blood increases the frequency of activated CD11b+ CD15+ granulocytes which inhibit T cell function. J Immunol Methods. 2008 Nov 27;
  11. T-Cell Xtend™ mechanism of action. http://www.youtube.com/watch?v=VAsezk74TkE&feature=youtube_gdata.
  12. Koch MA, Tucker-Heard G, Perdue NR, Killebrew JR, Urdahl KB, Campbell DJ. The transcription factor T-bet controls regulatory T cell homeostasis and function during type 1 inflammation. Nat Immunol. 2009 Jun;10(6):595–602

PII: S0163-4453(10)00039-3

doi: 10.1016/j.jinf.2010.01.012

Journal of Infection
Volume 60, Issue 5 , Pages 344-350 , May 2010

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